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cd34 microbead kit ultrapure, human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd34 microbead kit ultrapure, human
    Cd34 Microbead Kit Ultrapure, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit+UltraPure%2C+human/custom%40130-100-453%4042603588
    Average 96 stars, based on 288 article reviews
    cd34 microbead kit ultrapure, human - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Temporal Lysophosphatidic Acid Supplementation Enhances Megakaryocyte Differentiation and Platelet Production From Human Hematopoietic Progenitors
    Article Snippet: Umbilical cord blood was diluted with phosphate buffer saline (PBS) at a 1:1 ratio, and gently layered onto Lymphoprep TM (STEMCELL Technologies, Canada), and then centrifuged at 2,000 rpm for 20 min with break. .. Mononuclear cells located between plasma and Lymphoprep TM layers were harvested, washed twice with PBS, and centrifuged at 1,500 rpm for 5 min. HSPCs were isolated from mononuclear cells using a CD34 MicroBead Kit (Miltenyi Biotec, Germany). ..

    Article Title: Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.
    Article Snippet: Cells from a leukapheresis sample were isolated using Ficoll-Paque PLUS (GE Healthcare) and density centrifugation, followed by NH4Cl lysis to remove red blood cells. .. A secondary isolation step was completed using CD34 MicroBead Kit (Miltenyi Biotec) performed according to the manufacturer’s protocol for positive selection of CD34+ cells from the mononuclear population. .. Isolated CD34+ cells were cultured in expansion medium (Stemspan SFEM (Stem Cell Technologies 09650), 50ng/mL rhFLt3L (R&D 308-FKN), 50ng/mL rhSCF (R&D 255-SC), 10ng/mL rhIL-3 (R&D 203-IL), 10ng/mL rhIL-6 (R&D 206-IL), 35nM UM171 and 500nM Stemreginin) with or without SA for 4 and 7 days.

    Article Title: Dysregulated HELLS expression alters cellular processes and serves as a potential prognostic marker in acute myeloid leukemia.
    Article Snippet: In vitro and ex vivo cell culture and myeloid differentiation: AML cell lines, HL-60 (CCL-240) and U937 (CRL-1593.2), were procured from the American Type Culture Collection (ATCC) and maintained under the culture conditions recommended by ATCC (www.atcc.org). .. MNCs were isolated from whole blood using Lymphoprep (Stem Cell Technologies) and from 30-50 mL of cord blood or 1-2 mL of AML patient-derived bone Jo urn al Pr eroo f marrow cells, followed by isolation of CD34+ HSPCs and LSPCs using the CD34 microbead kit (Miltenyi Biotech, 130046703) per the manufacturer’s protocol. .. The isolated HSPCs were cultured in ex vivo media (Lonza, 04-743Q) supplemented with the recommended concentrations of cytokines such as IL3 (10 ng/mL), IL6 (10 ng/mL), SCF (100 ng/mL), TPO (20 ng/mL), and FLT3-L (100ng/mL).

    Article Title: Wild-Type p53 Protein Enhances APR-246-Induced Cytotoxicity in Acute Myeloid Leukemia and Normal Hematopoietic Stem/Progenitor Cells
    Article Snippet: .. CD34 + cells were isolated using the CD34 Microbead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Briefly, for positive selection, 1 × 10 8 cells were resuspended in 300 μL of 10% FBS and incubated with 100 μL of FcR blocking buffer and 100 μL of CD34 microbeads (Miltenyi Biotec, 130-100-453) for 30 min in the refrigerator at 4 °C.

    Selection:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.
    Article Snippet: Cells from a leukapheresis sample were isolated using Ficoll-Paque PLUS (GE Healthcare) and density centrifugation, followed by NH4Cl lysis to remove red blood cells. .. A secondary isolation step was completed using CD34 MicroBead Kit (Miltenyi Biotec) performed according to the manufacturer’s protocol for positive selection of CD34+ cells from the mononuclear population. .. Isolated CD34+ cells were cultured in expansion medium (Stemspan SFEM (Stem Cell Technologies 09650), 50ng/mL rhFLt3L (R&D 308-FKN), 50ng/mL rhSCF (R&D 255-SC), 10ng/mL rhIL-3 (R&D 203-IL), 10ng/mL rhIL-6 (R&D 206-IL), 35nM UM171 and 500nM Stemreginin) with or without SA for 4 and 7 days.

    Article Title: Human haematopoietic stem cells remember inflammatory stress
    Article Snippet: Mononuclear cells from pools of male and female CB (approximately 1–8 donors) were obtained by centrifugation over a density barrier of Lymphoprep (Multicell). .. After red cell lysis with ammonium chloride (STEMCELL technologies), mononuclear cells were enriched for HSPCs by positive selection with a CD34 Microbead kit (Miltenyi) per the manufacturer’s instructions, or by lineage depletion as previously described . ..

    Magnetic Cell Separation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Cell Culture:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Foxs1-mediated transformation of CD34 + fibroblast to myCAFs promotes tumor growth.
    Article Snippet: .. These cells were purified with a Cd34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured in S/P cell culture medium. ..

    Ex Vivo:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Purification:

    Article Title: Foxs1-mediated transformation of CD34 + fibroblast to myCAFs promotes tumor growth.
    Article Snippet: .. These cells were purified with a Cd34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured in S/P cell culture medium. ..

    Clinical Proteomics:

    Article Title: Temporal Lysophosphatidic Acid Supplementation Enhances Megakaryocyte Differentiation and Platelet Production From Human Hematopoietic Progenitors
    Article Snippet: Umbilical cord blood was diluted with phosphate buffer saline (PBS) at a 1:1 ratio, and gently layered onto Lymphoprep TM (STEMCELL Technologies, Canada), and then centrifuged at 2,000 rpm for 20 min with break. .. Mononuclear cells located between plasma and Lymphoprep TM layers were harvested, washed twice with PBS, and centrifuged at 1,500 rpm for 5 min. HSPCs were isolated from mononuclear cells using a CD34 MicroBead Kit (Miltenyi Biotec, Germany). ..

    Lysis:

    Article Title: Human haematopoietic stem cells remember inflammatory stress
    Article Snippet: Mononuclear cells from pools of male and female CB (approximately 1–8 donors) were obtained by centrifugation over a density barrier of Lymphoprep (Multicell). .. After red cell lysis with ammonium chloride (STEMCELL technologies), mononuclear cells were enriched for HSPCs by positive selection with a CD34 Microbead kit (Miltenyi) per the manufacturer’s instructions, or by lineage depletion as previously described . ..



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    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed <t>progenitor</t> <t>cells</t> (A) Flow cytometric analysis of <t>CD34,</t> c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted <t>CD34</t> + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .
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    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed <t>progenitor</t> <t>cells</t> (A) Flow cytometric analysis of <t>CD34,</t> c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted <t>CD34</t> + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .
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    Miltenyi Biotec human cd34 microbead kit ultrapure
    NOX1i in Myeloid-HIS-HCC mice reduces monocyte and tumor-associated macrophage infiltration. (A) Schematic overview of the experimental set-up. Male NSG-SGM3 mice (n = 11) were sublethally irradiated and the immune system was partly reconstituted using human cord blood (CB)-derived hematopoietic stem and <t>progenitor</t> <t>cells</t> (HSPCs). Four weeks post humanization, orthotopic human HCC was induced and mice were treated with 50 µM ML171 (NOX1i; n = 6) or vehicle (n = 5), twice per week for 3 weeks. (B) Human-to-murine immune cell (CD45) chimerism in the indicated tissues. (C) Human engraftment based on the number of human CD45 cells. Data are represented as violin plots showing median and quartiles. (D,E) Human immune cell subsets (D) and monocyte subsets (E) in indicated tissues represented as percentage of human CD45 cells. (F) Number of human macrophages and monocytes in the indicated tissues. (G) Representative immunohistochemistry images of infiltrated human monocytes/macrophages in liver tissue of HCC-bearing humanized mice. Sections were stained for human CD14 (brown), or matched IgG control. Scale bars: 100 µm. (B,D,E) . Data are shown as mean (SD). (C–E) Adjusted p-values were calculated using multiple unpaired t-test with Holm-Šídák correction (D,E) , or p-values were calculated using unpaired t-test with Welch’s correction or unpaired Mann-Whitney U test based on normal distribution (C,E) . *p < 0.05. HCC: hepatocellular carcinoma, NOX1i: NOX1 inhibition, huCD45: human CD45, muCD45: murine CD45, NK = natural killer, DCs: dendritic cells.
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    ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human <t>CD34</t> + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).
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    ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human <t>CD34</t> + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).
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    Image Search Results


    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Journal: Cell Reports Medicine

    Article Title: ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development

    doi: 10.1016/j.xcrm.2026.102879

    Figure Lengend Snippet: B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Article Snippet: Leukocytes from UCB and mPB were first purified using the Erythrocyte Sedimentation Kit II (Miltenyi), followed by CD34 + cell enrichment with the CD34 MicroBead Kit UltraPure (Miltenyi) per manufacturer’s instructions.

    Techniques: Expressing

    NOX1i in Myeloid-HIS-HCC mice reduces monocyte and tumor-associated macrophage infiltration. (A) Schematic overview of the experimental set-up. Male NSG-SGM3 mice (n = 11) were sublethally irradiated and the immune system was partly reconstituted using human cord blood (CB)-derived hematopoietic stem and progenitor cells (HSPCs). Four weeks post humanization, orthotopic human HCC was induced and mice were treated with 50 µM ML171 (NOX1i; n = 6) or vehicle (n = 5), twice per week for 3 weeks. (B) Human-to-murine immune cell (CD45) chimerism in the indicated tissues. (C) Human engraftment based on the number of human CD45 cells. Data are represented as violin plots showing median and quartiles. (D,E) Human immune cell subsets (D) and monocyte subsets (E) in indicated tissues represented as percentage of human CD45 cells. (F) Number of human macrophages and monocytes in the indicated tissues. (G) Representative immunohistochemistry images of infiltrated human monocytes/macrophages in liver tissue of HCC-bearing humanized mice. Sections were stained for human CD14 (brown), or matched IgG control. Scale bars: 100 µm. (B,D,E) . Data are shown as mean (SD). (C–E) Adjusted p-values were calculated using multiple unpaired t-test with Holm-Šídák correction (D,E) , or p-values were calculated using unpaired t-test with Welch’s correction or unpaired Mann-Whitney U test based on normal distribution (C,E) . *p < 0.05. HCC: hepatocellular carcinoma, NOX1i: NOX1 inhibition, huCD45: human CD45, muCD45: murine CD45, NK = natural killer, DCs: dendritic cells.

    Journal: Frontiers in Pharmacology

    Article Title: Harnessing human immune system models to validate NADPH oxidase 1 inhibition as treatment for hepatocellular carcinoma

    doi: 10.3389/fphar.2026.1808515

    Figure Lengend Snippet: NOX1i in Myeloid-HIS-HCC mice reduces monocyte and tumor-associated macrophage infiltration. (A) Schematic overview of the experimental set-up. Male NSG-SGM3 mice (n = 11) were sublethally irradiated and the immune system was partly reconstituted using human cord blood (CB)-derived hematopoietic stem and progenitor cells (HSPCs). Four weeks post humanization, orthotopic human HCC was induced and mice were treated with 50 µM ML171 (NOX1i; n = 6) or vehicle (n = 5), twice per week for 3 weeks. (B) Human-to-murine immune cell (CD45) chimerism in the indicated tissues. (C) Human engraftment based on the number of human CD45 cells. Data are represented as violin plots showing median and quartiles. (D,E) Human immune cell subsets (D) and monocyte subsets (E) in indicated tissues represented as percentage of human CD45 cells. (F) Number of human macrophages and monocytes in the indicated tissues. (G) Representative immunohistochemistry images of infiltrated human monocytes/macrophages in liver tissue of HCC-bearing humanized mice. Sections were stained for human CD14 (brown), or matched IgG control. Scale bars: 100 µm. (B,D,E) . Data are shown as mean (SD). (C–E) Adjusted p-values were calculated using multiple unpaired t-test with Holm-Šídák correction (D,E) , or p-values were calculated using unpaired t-test with Welch’s correction or unpaired Mann-Whitney U test based on normal distribution (C,E) . *p < 0.05. HCC: hepatocellular carcinoma, NOX1i: NOX1 inhibition, huCD45: human CD45, muCD45: murine CD45, NK = natural killer, DCs: dendritic cells.

    Article Snippet: Freshly isolated CB-derived PBMCs, isolated from CB as described for buffy coat, were enriched for HSPCs based on positive immunomagnetic selection of CD34 + expressing cells using the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, #130–100-453) according to manufacturer’s guidelines.

    Techniques: Irradiation, Derivative Assay, Immunohistochemistry, Staining, Control, MANN-WHITNEY, Inhibition

    ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human CD34 + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).

    Journal: Nucleic Acids Research

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch

    doi: 10.1093/nar/gkag613

    Figure Lengend Snippet: ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human CD34 + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).

    Article Snippet: CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ].

    Techniques: Staining